Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in mouse perinatal development

Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function. BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect reproductive organ development, brain development, metabolic disease and post-natal behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane) and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this effective metabolizing system is weak in the fetus. In the present study, we demonstrated that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the fetus and newborn pups, and was increased to the same level as BPA post-partum. In conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism during the perinatal period, suggesting that the metabolism of individual Bisphenol analogs requires assessment to properly gauge their risks.     

苜蓿地撂荒过程中土壤-微生物-胞外酶及化学计量的变化

紫花苜蓿(Medicago sativa L.)作为优良修复草种在黄土高原广泛种植,然而随着农村人口迁移及土地利用变化,苜蓿人工草地大量弃置的问题已引起广泛关注。为研究苜蓿弃置草地土壤质量变化规律,本研究分析了不同弃置年限(0年、5年和10年)苜蓿草地土壤养分、微生物量、参与土壤碳氮磷(C,N,P)循环的胞外酶活性及其化学计量变化。结果表明：总体上,土壤和微生物C,N含量随弃置年限呈增加趋势,而C,N相关胞外酶活性和化学计量变化幅度较小;土壤和微生物P含量随弃置年限增加而显著降低,但P相关胞外酶活性及化学计量均有不同程度的增加。进一步分析发现：相较于其他土层,表层(0～20 cm)土壤C,N,P含量及其酶活性较高,其变化趋势与总体结果相似;亚表层土壤(20～40 cm)和(80～100 cm)深层土壤C,N,P含量及其酶活性呈先增后减趋势,且各指标较于表层土壤均降低40%～80%。相关分析表明弃置草地土壤由于P匮乏而逐步退化,可通过补施磷肥改善。     

Increased abundance of patatins,lipoxygenase and miraculins in a thaxtomin A-habituated potato Russet Burbank somaclone with enhanced resistance to common scab

Potato common scab caused by the actinobacterium Streptomyces scabiei is characterized by the formation of corky lesions on tubers that reduce their marketability. Management of common scab is very complex and often ineffective under various environmental conditions. Using potato varieties that are more resistant to common scab remains one of the most efficient strategies to control this disease. However, very little is known about the factors associated with resistance to common scab. Somaclone RB9 regenerated from thaxtomin A-habituated potato Russet Burbank calli produced tubers more resistant to common scab than the original variety. Comparison of the RB9 tuber proteome with that of Russet Burbank using label-free quantitative proteomic analysis revealed changes in the accumulation of defence-related proteins from the patatin and lipoxygenase (LOX) families, which are involved in the metabolism of lipids, and of two miraculins of the Kunitz-type protease inhibitors family. The implication of LOX during common scab infection was studied using synchronized minitubers developed from leaf-bud cuttings. S. scabiei infection stimulated the accumulation of LOX in both Russet Burbank and RB9 minitubers, but this accumulation was intensified in RB9 minitubers. Infection also increased LOX activity in Russet Burbank and RB9 minitubers. However, LOX activity measured in noninfected RB9 minitubers was similar to that of infected Russet Burbank minitubers, indicating endogenous activation of LOX activity in RB9 minitubers. We discuss how increased LOX abundance and activity in the somaclone RB9 may contribute to improving tuber defence against common scab.     

不同种植年限苜蓿地土壤理化性状

本研究分析了半干旱区不同种植年限苜蓿(Medicago sativa)地土壤理化性状。结果表明,随着苜蓿种植年限的增加,0-100 cm土层土壤容重出现不规律的波动,苜蓿地土壤容重值整体偏大;土壤有机质随种植年限的增加而增大,随土层深度增加而显著减小(P0.01),并且在种植5年的苜蓿地中提高最明显,在种植8年的苜蓿地中最低。各年限苜蓿地均表现出土壤有机质的"表聚性"。在一定的种植年限范围内,种植苜蓿对土壤有机质具有累积作用;土壤全氮含量随苜蓿种植年限的增长表现为先增大后减小的趋势,随土层深度的加深而逐渐减小,种植苜蓿4年时土壤全氮含量最大,8年时苜蓿地土壤全氮含量最低,并且土壤全氮含量主要集中于0-40 cm土层,全氮显著累积作用于表层土壤;土壤硝态氮随着苜蓿种植年限的增加而先增加后减小,随着土层的加深而逐渐降低,表层土壤对土壤硝态氮有累积作用,并且与苜蓿种植时间长短有显著相关性(P0.05)。而铵态氮含量在剖面上变化不大,与苜蓿生长年限没有显著相关性(P0.05)。今后需从栽培苜蓿草地的管理措施上深入探讨栽培苜蓿地的最佳种植和利用年限。     

7株牛源A型巴氏杆菌的分离鉴定及耐药性分析

对2014年沈阳地区患有呼吸系统疾病的育肥牛场进行病原分离,得到7株分离菌,对其进行培养形态及染色观察、病理组织切片观察、动物致病性观察及16S序列分析,初步鉴定为牛多杀性巴氏杆菌(Pm)。利用Pm种特异性引物及荚膜血清特异性引物进行PCR扩增,均得到目的基因条带。对分离菌进行药敏试验的结果显示,5株分离菌株已对阿米卡星、新诺明产生较强耐药性;7株分离菌均对氟苯尼考、恩诺沙星、四环素及氨苄西林敏感。由此确定,分离菌株均为牛荚膜A型巴氏杆菌,且已具有一定耐药性。     

几种热带相思树种的扦插育苗试验

几种热带相思树种经大田扦插育苗试验结果表明：在广州地区以秋季进行萌条扦插育苗效果较好。以马占相思、直干大叶相思、厚荚相思萌条作插穗，用１／３火烧土加２／３黄心土作基质，蘸上自配的ＩＢＤ２＃多效生根粉，直接插入育苗容器育苗，苗床用塑料薄膜拱棚加盖遮光度为６０％的黑网以调节控制温湿度及光照，在基质和插穗不消毒、定期淋浇１‰的复合肥水的情况下，扦插成活率分别达６１．８％、８８．８％和６８．９％。其中直干大叶相思扦插易成活且受季节影响较小。     

林火对土壤氮含量的扰动作用

通过对大兴安岭地区新林林业局 2 0a内不同年份间隔的 11块火烧迹地土壤进行调查取样和室内实验分析 ,发现火烧后不同年限、不同强度的火烧迹地 ,土壤全氮与有效氮含量变化情况如下 :低强度火烧后 9d ,土壤全氮和有效氮含量均比未烧前低 ,这与中、高强度火烧后的情况正好相反。火烧后 4a内 ,土壤氮的含量基本都比未烧林地有所下降 (中强度火烧迹地土壤有效氮含量除外 )。以后有逐年增加的趋势。火烧迹地降第一场雨后 ,低强度火烧迹地土壤全氮比降雨前减少 ,有效氮增加 ;中强度火烧迹地土壤全氮和有效氮均比降雨前增加 ;高强度火烧迹地土壤全氮和有效氮均比降雨前有所下降。连年火烧迹地土壤全氮减少 ,有效氮增加     

百山祖冷杉异砧嫁接试验初报

百山祖冷杉天然更新困难，为解决这种冷杉的繁殖更新问题，采用以日本冷杉为砧木的异砧嫁接试验，初获成功。     

从澳大利亚引进的两种相思植物光合速率和蒸腾效率的初步研究

以LCA-4光合蒸腾测定系统进行大腺相思和多花相思叶片气体交换过程的测定。结果如下：（1）净光合速率日变化明显，且变化趋势大体一致，即10：00-13：00具有较高的光合速率，其它测定时间相对较低。（2）两树种净光合速率存在差异。大腺相思高于多花相思，其最大值分别为19.6(10:00),16.1(13:00)μmolm^-2s^-1，日平均值分别为11.9,9.8μmolm^-2s^-1，反映了大腺相思的生长速度较多花相思快。（3）大腺相思和多花相思蒸腾速率日变化明显，正午前后较高，13：00达到最大，日平均值多花相思略高于大腺相思，分别为3.3,3.0mmolm^-2s^-1，表明在相同的环境条件下大腺相思的耗水量低于多花相思。（4）两树种蒸腾效率的日变化趋势大体上也类似，即在9：00-11：00较高，此期间大腺相思和多花相思和多花相思的平均值分别为5.51,4.21μmolCO2/mmolH2O。日平均值大腺厢思和多花相思分别为3.84,2.88μmolCO2/mmolH2O,表明消耗等量的水分大腺相思比多花相思能固定更多的CO2量，即有更高的水分利用效率。     

旱薄山地优良肉用杏品种的引种研究

1990年－1999年，在太行山西侧旱薄山地进行了杏树的引种与选育，通过对13个杏品种树体生长量，产量及果实品质的分析，得出阳坡山地宜栽植大黄甜，华县大接杏，大杏梅，银白杏，海东杏；石灰岩山地宜发展大黄甜，大杏梅，华县大接杏;土层较厚，有施肥条件的沟块地宜发生兰州大接杏，山黄杏。